Equipment available to users

A Bio-Rad iQ5 is available to users for real-time PCR experiments. It enables to monitor PCR product accumulation on line during the PCR reaction in presence of a fluorescent dye (SYBR Green) or of fluorescently labeled oligonucleotide probes (Hybridization probes). The Bio-Rad iQ5 uses 96-well reaction format and up to five targets can be multiplexes in one well. Information regarding how to access the real-time PCR instrument and tips for running a quatitative real-time PCR experiment please refer to 'Instructions and tips for using the the RT-PCR'

The Eppendorf epMotion 5075 automated liquid handling systems automates routine pipetting tasks. This accurate pipetting stations helps eliminate manual pipetting errors and maximizes the reproducibility of assays.
The system is set up for:
     - DNA extractions
     - Setting up PCR reactions
     - Pooling of PCR reactions from several microtiter plates
     - Preparation of Illumina libraries

The Agilent Technologies 4200 TapeStation is a microfluidics electrophoresis system that provides sizing and quantitation of DNA and RNA samples and displays results in a digital format. Sizing range:
     - RNA: 100-6000nt
     - DNA: D1000: 35-1000bp; D5000: 100-5000bp
     - Genomic DNA:  200-60,000bp
More information about the tapes and sample submission can be found in this document.
To submit samples download the sample submission form and submit it electronically to the MCIC.

The Sage Science Pippin Prep is used for targeted size selection of DNA libraries for Illumina or Ion Torrent sequencing. The instrument allow for  collect narrow and even fragment distributions between 100bp – 1.5kb. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination.

The Covaris S220 Focused-Ultrasonicator patented operates at high frequencies (500KHz) and uses Adaptive Focused Acoustics Tubes and and advanced electronics to deliver controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml.  It's used for DNA, RNA, and chromatin shearing to specific sizes, tissue homogenization, cell lysis, compound dissolution, and particle micronization.

The Qubit Fluorimeter 2.0 and Qubit Assay Kits (Sage Science) allow for accurate measure of DNA or RNA oncentrations by detecting target specific fluorescence. The DNA or RNA samples is mixed with the Qubit Assay Kit reagents containing dyes that fluoresce only when bound to nucleic acids and the concentration is read in the fluorimeter. The assay is performed at room temperature, and the signal is stable for up to 3 hours. Different Qubit Assay Kits are available for dsDNA, oligonucleotide, RNA, microRNAs and different sensistivity/concentrations rangees.

The NanoDrop 2000 is a UV and visible spectrophotometer used to measure the concentration and purity of DNA, RNA and protein samples. Requires only 0.5 – 2 µl of sample and calculates sample purity ratios (260/280 nm and 260/230 nm).