Applied Biosystems SeqStudio Genetic Analyzer is a low-throughput benchtop system that delivers Sanger sequencing and fragment analysis. This is a reliable easy to use instrument with an all-in-one cartridge with a single polymer for sequencing and fragment analysis applications.
Sanger sequencing uses the 3'-BigDye dideoxynucleotide triphosphates labeling chemistry. It requires a DNA template, a sequencing primer, a thermal stable DNA polymerase, nucleotides (dNTPs), fluorescently labeled 3'-BigDye dideoxynucleotides (ddNTPs), and buffer. The components are combined in a reaction that is subjected to cycles of annealing, extension, and denaturation in a thermal cycler. Thermal cycling the sequencing reactions creates and amplifies extension products that are terminated by one of the four dideoxynucleotides. The ratio of deoxynucleotides to dideoxynucleotides is optimized to produce a balanced population of long and short extension products.
To submit samples for sequencing